بررسی تاثیر روغن سیاه دانه بر ساختار نفرون های کلیوی در موش های نژاد NMRI تیمار شده با دوز بالای نانوذرات نقره

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه زیست شناسی، دانشکده علوم پایه، دانشگاه اراک، اراک، ایران

چکیده

امروزه با افزایش استفاده از نانوذرات نقره در محصولات مختلف ازجمله محصولات مصرفی و پزشکی نگرانیهای جدی
درمورد خطرات بالقوه نانو ذرات نقره ابراز شده است، هدف از این مطالعه بررسی اثر حفاظتی روغن سیاه دانه (NSO) به
عنوان یک آنتی اکسیدان قوی بر تغییرات هیستولوژیکی بافت کلیه و پارامترهای بیوشیمیایی خون در موش های نر تیمار
شده با دوز بالای نانو ذرات نقره ) AgNPs ( بود.
24 سر موش نر بالغ نژاد NMRI با میانگین وزنی 25 تا 30 گرم به طور تصادفی به 4 گروه 6 تایی شامل گروه کنترل،
AgNPs ( mg/kg/day500 ،) NSO ( ml/kg/day 5 ( و AgNPs + NSO تقسیم و به صورت دهانی برای 35 روز تیمار شدند.
پس از اتمام دوره تیمار، موش ها تشریح، کلیه چپ آنها خارج ،فیکس، قالب گیری، برش گیری و پاساژ بافتی انجام شد و
با استفاده از روش هایدن هان – آزان رنگ آمیزی گردید. پارامترهای بافت کلیه با روش استریولوژی مورد ارزیابی قرار
گرفت. نمونهی سرم خون نیز آنالیز شد. داده ها با روش آنالیز واریانس یک طرفه و آزمون Tukey بررسی و تفاوت
میانگین ها در حد) 05 / 0P< ( معنی دار در نظر گرفته شد.
در این پژوهش افزایش معنیداری در میانگین حجم کل جسمک کلیوی، گلومرول، تافت، غشای کپسول بومن و کاهش
معنی داری در میانگین حجم کل فضای کپسول بومن در گروه AgNPs نسبت به گروه کنترل مشاهده شد. میزان
مالون دیآلدئید و اوره سرم نیز در گروه AgNPs در مقایسه با کنترل افزایش معنی داری داشت ) 05 / 0P< ( پارامتر های
ذکر شده در گروه AgNPs + NSO در سطح گروه کنترل نرمال شد.
نتایج ما نشان داد که نانوذرات نقره می تواند اثرات نامطلوبی بر ساختار گلومرول داشته باشد و روغن سیاه دانه می تواند
این اثرات را جبران کند.

کلیدواژه‌ها


عنوان مقاله [English]

The effects of Nigella sativa oil on kidney nephron structure NMRI mice treated with high dose of silver nanoparticles

نویسندگان [English]

  • SMA shariatzadeh
  • Afsaneh Nemati
Department of Biology, Faculty of Science, Arak University, Arak, Iran
چکیده [English]

Today, with the increasing use of silver nanoparticles in consumer products and medical products, including serious concerns have been expressed about the potential risks of nano-silver particles, the goal of this study was to evaluate the protective effects of Nigella sativa oil (NSO) as a potent antioxidant on changes histological kidney tissue and blood biochemical parameters in rats treated with high dose of silver nanoparticles (AgNPs), respectively. 24 adult male rats (NMRI) with an average weight of 25 to 30 g were randomly assigned to 4 groups of 6 rats including the control group, AgNPs (mg / kg / day500), NSO (ml / kg / day 5) and AgNPs + NSO split and both were treated orally for 35 days. At the end of the treatment period, mice, anatomy, left kidney removed, fix, molding, cutting and tissue processing was carried out using -Zan Han Hayden were stained. Renal tissue was evaluated parameters stereologically. Serum samples were analyzed. ANOVA and Tukey test to evaluate the study data. The differences in the extent (05/0 P <) were considered significant.
In this study, a significant increase in mean total volume of renal corpuscle, glomeruli, Taft, Bowman's capsule membrane and a significant reduction in the volume of the entire space of Bowman's capsule AgNPs group compared to the control group. Malvndy‌Ldyyd rate and serum urea AgNPs compared to the control group showed a significant increase (05 / 0P <) the parameters listed in Group AgNPs + NSO in the control group was normal.

کلیدواژه‌ها [English]

  • Biochemical parameters
  • Black oil seeds
  • kidney
  • Mice
  • Silver
  • Stereology
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