بهبود فعّالیّت آنزیم‌های آنتی‌اکسیدان گیاه ذرّت (Zea mays L.) پس‌از آبیاری با آب مغناطیده

نوع مقاله: مقاله پژوهشی

نویسندگان

بخش علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

گرچه مکانیسم اصلی اثر میدانهای مغناطیسی برگیاهان هنوز به طور کامل مشخص نیست، اما شواهد موجود حاکی از
دخالت این میدانها در افزایش مقدار گونههای فعّال اکسیژن و نیز فعالیت سیستم آنتیاکسیدانها در سلولهای گیاهی
است. دراین تحقیق،تأثیر میدان مغناطیسی بر ویژگیهای فیزیکی و شیمیایی آب و اثر آبیاری با آب مغناطیده )پساز
عبور از یک میدان مغناطیسی 110 میلیتسلا( برفعّالیّت سیستم آنتیاکسیدان گیاه ذرّت ) Zea mays L. ( بررسی شد.
طیفهای رامان و FTIR آب قبل و بعداز قرارگرفتن درمعرض میدان مغناطیسی مقایسه شد. طیف رامان آب مغناطیده
باگروه کنترل تفاوتی نداشت، اما طیفهای FTIR آن افزایش شدت جذب را نشان داد. نتایج نشان میدهد که میدان
مغناطیسی با افزایش درجهی آزادی پیوند های هیدروژنی سبب کاهش کشش سطحی شدهاست. درنتیجه، میزان حلالیّت
آب افزایش یافتهاست. نتایج با افزایش فعّالیّت آنزیمهای کاتالاز و آسکوربات پراکسیداز و نیز افزایش میزان آنتوسیانینها
در گیاهان تیمار شده با آب مغناطیده، نسبت به گروه شاهد معنیدار بود. افزایش فعّالیّت این دو آنزیم و افزایش
آنتوسیانین همراه با عدم تغییر فعّالیّت آنزیم سوپراکسید دیسموتاز که خود سبب تولید هیدروژن پراکسید میکند، سبب
ممانعت از آسیب رسیدن به غشا و کاهش پراکسیداسیون لیپیدهای آن میشود. عدم افزایش آنزیم پراکسیداز نیز با حفظ
انعطاف پذیری دیوارهها سبب حفظ توان رشد در سلولهای گیاهان تیمار شده با آب مغناطیده شد.

کلیدواژه‌ها


عنوان مقاله [English]

Improvement of Antioxidant Enzymes Activity of Zea mays L. After Treatment with Magnetized Water

نویسندگان [English]

  • Maryam Soleymani
  • Faezeh Ghanati
  • Somayeh Mohamadalikhani
  • Hasan Zare maivan
Department of Plant Science, Faculty of Biology Science, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Although the exact mechanism(s) of the effects of magnetic fields on plants is not completely understood, available evidences suggest that the effects of these fields are mediated by reactive oxygen species (ROS) and activity of antioxidant system. In the present study, the effects of a magnetic field (110 mT) on physical and chemical properties of water, and the effect of magnetized water on the antioxidant system activity of maize plants were investigated. Raman and FTIR absorption spectra of water before and after magnetization were compared. While the Raman spectrum of magnetized and normal water were identical, the intensity of FTIR absorption spectrum of magnetized water was significantly increased, suggesting changes in the distribution of hydrogen bound among water molecules and increase of its solubility. In comparison with the control plants, irrigation with magnetized water resulted in the increase of antioxidant enzymes activity i.e., SOD and CAT as well as increase of the contents of non-enzymatic compounds e.g., anthocyanins of the plants. In addition, lower amounts of H2O2, and lower activity of POD was observed in magnetized water-irrigated plants, compared to the control group. All together, these changes resulted in the maintenance of membrane integrity and improvement of growth and development of those plants which were irrigated with magnetized water.

کلیدواژه‌ها [English]

  • Antioxidant Activity
  • magnetic field
  • magnetized water
  • Maize
  • Raman spectrum
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