مقایسه اثر عصاره آبی الکلی رزماری روی بقای سلول‌های سرطانی سر و گردن رده HN5 و سلول‌های پیش ساز عصبی موش سوری

نوع مقاله: مقاله پژوهشی

نویسندگان

زیست شناسی، علوم، شهید چمران اهواز

چکیده

سرطان یک مشکل اصلی سلامتی در تمام دنیاست و سالانه تعداد بیماران سرطانی افزایش مییابد. به علت مقاومت دارویی، درمان سرطان نیازمند تولید داروهای ضد سرطان جدید است. رزماری از جمله گیاهان دارویی است که اثرات ضد سرطانی آن گزارش شده است. هدف مطالعه حاضر بررسی اثر عصاره رزماری روی بقا سلول در HN5 های سرطانی مقایسه با سلولهای پیش ساز عصبی موش سوری است. سلولهای پیش ساز عصبی از جنین های موش سوری باردار روزه با روش هضم آنزیمی استحصال شد. این سلولها و سلولهای پیش ساز عصبی و سلول 17 با غلظتهای HN5 های ساعت تیمار شدند و میزان بقای 72 و 48 ،24 میکروگرم بر میلی لیتر عصاره رزماری به مدت 500 و 200 ،100 ،50 اندازه MTTآنها با آزمون گیری شد. نتایج حاصل نشان داد که عصاره رزماری در غلظت میکروگرم 500 و 200 ،100 های را کاهش داد که در مقایسه با اثر آن روی سلول HN5 بر میلی لیتر بقای سلولهای
های پیش ساز عصبی معنیدار بود ). عصاره در غلظتP<0/05( میکروگرم بر میلی لیتر تکثیر سلول 100 و 50 های های پیش ساز عصبی را افزایش داد ). عصاره رزماری میP<0/05( میکروگرم بر میلی لیتر بقای آنها را کاهش داد500) و در غلظت P<0/05( تواند به صورت وابسته به دوز و زمان بقای سلولی را در سلول های سرطانی کاهش و در سلول های پیش ساز عصبی افزایش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Comparison of Rosemary (Rosmarinus officinalis L.) hydroalcoholic extract on the viability of head and neck cancer cells Line HN5 and neuronal progenitor cells of Mouse

نویسندگان [English]

  • Tayebeh Mohammadi
  • Elham Hoveizi
Biology, Sciences, Shahid Chamran of Ahvaz
چکیده [English]

Cancer is a main health problem worldwide and the number of cancer patients is increasing annually. Cancer treatment needs new anticancer medicines because of drug resistance. Rosemary is one of the herbal medicines which its anti-cancer effects have been reported. The purpose of the present study was to evaluate rosemary extract effect on HN5 cancer cells viability in comparison with neuronal progenitor cells(NPCs). NPCs were obtained from 17 days pregnant mice by enzymatic digestion method. These cells and HN5 cells were treated with 50, 100, 200 and 500 µg/ml of rosemary extract for 24, 48 and 72 hours. Their viability was measured using MTT assay. Results showed that rosemary extract decreased HN5 cells viability in 100, 200 and 500 µg/ml concentrations which were significant in comparison with NPCs. The extract increased NPCs proliferation rate in 50 and 100 µg/ml concentration and decreased their viability in 500 µg/ml concentration. Rosemary extract can decrease cell viability as a dose and time dependent manner but this effect also depends on the cell type such as its killing effect on cancer cells was more. 

کلیدواژه‌ها [English]

  • cancer
  • cell viability
  • PNCs
  • Rosemary
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