2گروه زیست شناسی،دانشکده علوم پایه، واحد تهران شمال ،دانشگاه آزاد اسلامی واحد تهران شمال ، تهران، ایران.
چکیده
اسفناج (Spinacia oleracea L .) گیاهی یکساله متعلق به خانواده Chenopodiace است که سابقه کشت آن در ایران به دو هزار سال می رسد.هدف از انجام این پژوهش بررسی تغییرات ساختار آناتومی گیاه اسفناج در بخش متراکم از هوای آلوده شهر اراک ،بوده است. نمونه های بذر گیاه اسفناج بطور همزمان در دو گروه شاهد و تحت تیمار در دو بخش با هوای سالم پارک ملی هفتاد قله اراک و در بخش متراکم پارک ایرالکو کارخانه آلومینیوم از هوای آلوده شهر مورد کشت واقع شدند .بعد از رویش برگ های لپه ای و برگ های اصلی گیاه نمونه ها در محلول گلیسیرین الکل تثبیت شدند . بعد از برش گیری و رنگامیزی مضاعف با آبی متیل و کارمن زاجی ،توسط فتومیکروسکوپ عکسبرداری و مورد بررسی تغییرات ساختار تشریحی قرار گرفتند . نتایج حاصل از بررسی تغییرات ساختار تشریحی در برگ های گیاه اسفناج حاکی از آن بود که در نمونه هایی که در بخش متراکم از هوای آلوده شهر رویش پیدا کرده بودند نسبت به نمونه های شاهد، کوتیکول ضخامت بیشتری یافته و دستگاه کرک محافظتی به فراوانی یافت شد.ساختار کلرانشیم بی نظم شده و بافت پارانشیم ذخیره ایی مملو از رسوبات کریستالی اگزالاتی شده بودند. در نهایت میتوان نتیجه گرفت که نمونه های گیاهی مربوط به هوای آلوده شهر، دچار استرس ناشی از آلودگی هوا بودند که به احتمال زیاد بر کیفیت غذایی این گیاه نیز اثرات مخربی داشته است.
Influence of air pollution in part of Arak city on anatomical structure of spinach (Spinacea oleracea L.)
نویسندگان [English]
zohreh jafari1؛ Maryam Dehghan2
1Assistant professor, PhD in biology, Department of biology,Arak Branch, Islamic Azad university, Arak, Iran.
2Department of biology, North Tehran Branch, Islamic Azad university, Tehran, Iran
چکیده [English]
Background and Purpose: Spinach (Spinacia oleracea L.) is an annual plant belonging to the Chenopodiace family, whose cultivation history reaches two thousand years in Iran. The idea of this study was to investigate changes in the structure of spinach plant anatomy in the dense part of the contaminated air Arak city. Analysis method: Spinach seed samples simultaneously in two groups of control and treated, In two parts with clean air (Haftadgholeh Arak National Park )and condensation(Iralko Park Aluminum Factory) from air pollution in the city were planted. After growing Cotyledons leaves and Original leaves , the plant specimens were fixed in Alcohol -Gycerin solution . After sectioning and double coloring with Methylene blue and Zaghic Carmen, specimens were photographed by Photomicroscope then anatomical structure Changes were studied. Result and discussion: The changes in the anatomical structure of leaves of spinach showed that in samples that were grown in the dense portion of the city Compared to the control samples, had thicker cuticle and the structure of protective trichomes were abundant. Chlorenchyma have been irregular and stored parenchyma were full of Calcium oxalate crystal deposition. Finally, we can conclude that Plant samples of the air polluted city were stressed by air pollution which is likely have adversed effects on the quality of plant food.
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