ارزیابی اثر حفاظتی روغن سیاه دانه بر بافت تخمدان در موش های تیمار شده با نانو ذرات نقره

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه زیست شناسی، دانشکده علوم، دانشگاه اراک، اراک، ایران

چکیده

هدف: هدف از‌ این مطالعه بررسی اثر روغن سیاه دانه به‌عنوان یک آنتی‌اکسیدان کارآمد، بر بافت تخمدان در موش‌های بالغ تیمار‌شده با نانو ذرات نقره بود.
مواد ‌و ‌روش‌ها: در این مطالعه موش‌های ماده بالغ نژاد NMRI به 4 گروه (6=n): کنترل، نانوذرات نقره (300 میلی‌گرم بر کیلوگرم در روز)، عصاره سیاه دانه(5 میلی لیتر بر کیلوگرم در روز) و نانو ذرات نقره + عصاره سیاه دانه تقسیم شد. تیمار دهانی تا 30 روز انجام گرفت.‌ بعد ‌از‌ تیمار، تخمدان‌ها خارج و برای ارزیابی‌ هیستولوژیکی پردازش شد. پارامترهای مورفومتریک بافت تخمدان با استفاده از تکنیک استریولوژی تخمین زده شد. داده‌ها با استفاده از روش آنالیز واریانس یک‌طرفه ارزیابی شد و (05/0P<) از لحاظ آماری معنی‌دار در نظر گرفته‌ شد.
یافته‌ها: میانگین و آزمون توکی حجم کل تخمدان، کورتکس، مدولا و جسم‌زرد و نیز میانگین تعداد کل‌ فولیکول‌های بدوی، اولیه، ثانویه و گراف در گروه نانوذرات نقره در مقایسه با گروه کنترل به‌طور معنی‌داری کاهش یافت (001/0P<). به علاوه کاهش معنی‌داری در میانگین حجم اووسیت و هسته‌ آن در انواع مختلف فولیکول‌ها در گروه نانوذرات نقره نسبت به گروه کنترل مشاهده شد (001/0P<). تجویز همزمان روغن سیاه دانه و نانوذرات نقره اثرات نامطلوب نانوذرات نقره را بر پارامترهای فوق جبران کرد. از طرفی، میانگین تعداد انواع مختلف فولیکول‌ها‌ در موش‌های تیمار شده با روغن سیاه دانه نسبت به گروه کنترل به‌طور معنی‌داری افزایش یافت (001/0P<).
بحث: مصرف همزمان روغن سیاه دانه ونانوذرات نقره از اثرات نامطلوب نانوذرات نقره بر بافت تخمدان موش بالغ جلوگیری نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the protective effect of Nigella sativa oil on ovarian follicles in mice treated with silver nanoparticles

نویسندگان [English]

  • SMA shariatzadeh
  • Mozhgan Khagavi Jafarabad
Department of Biology, Faculty of Science, Arak University, Arak, Iran
چکیده [English]

Aim: the effect of Nigella sativa oil (NSO) as an efficient antioxidant on ovarian follicles following treatment with Silver Nanoparticles (SNP) in adult mice.
Material and methods: Twenty-four adult NMRI mice, weighing 27-30g were used in the present experiment. The animals were kept under optimum conditions of temperature (At 21± 2 ° C and ambient lighting conditions 12 hours of darkness and 12 hours of light) humidity and maintained on standard pellet diet with adequate water. They were divided into four groups of (six mice per group): control; Silver Nanoparticles (300 mg/kg/day, orally), Nigella sativa oil (5 ml/kg/day, orally) and finally Silver Nanoparticles plus Nigella sativa oil. After 30 days, the mice were sacrificed and the ovary tissues were separated. The results were analyzed using one-way ANOVA and Tukey’s test, and the means were significantly different at P<0.05.
Results: The results of this study showed that, the mean total volume of ovary and the number of primordial, primary, secondary and graph follicles significantly decreased in the Silver Nanoparticles group compared with the control group (p<0.05). Nigella sativa oil significantly increased the above parameters in the SNP +NSO group compared to silver nanoparticles group (p<0.05). Furthermore, the number of follicles and total volume of ovary significantly increased in the Nigella sativa oil treated mice compared to the control ones (p<0.05) (Table 1).
Conclusion: So it is not far-fetched that this oil has improved the factors in our study.

کلیدواژه‌ها [English]

  • silver nanoparticles
  • ovarian follicles
  • Nigella sativa oil
  • Mice
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